tsc1 floxed line  (Jackson Laboratory)

Journal: Scientific Reports

Article Title: Tsc1 ablation in Prx1 and Osterix lineages causes renal cystogenesis in mouse

doi: 10.1038/s41598-018-37139-9

Figure Lengend Snippet: Conditional knockout of Tsc1 in the Prx1 lineage resulted in the development of mTORC1-dependent cysts. ( A ) Prx1-Cre; Tsc1 f/f mouse kidney sections showed increased p-S6 signals. ( B ) Prx1-Cre; Tsc1 f/f mice showed enlarged kidneys that were rescued by rapamycin injection. Left panels: images of kidneys from different mouse lines. Right panel: the weights of the kidneys from different mouse lines. ( C ) H-E staining (40x) of the kidney sections of Prx1-Cre; Tsc1 f/f and control mice treated with rapamycin or vehicle. ( D ) H-E staining showed that 1- month-old mice did not develop cysts. ( E ) Images of kidney sections of Prx1-Cre; Tsc1 f/f and control mice treated with rapamycin or vehicle. ( F ) Cystic index of Prx1-Cre; Tsc1 f/f and control mice treated with rapamycin or vehicle. The cystic index for control mice is 0. ( G ) Quantitative analysis of glomerulus separation. N = 3. *P < 0.05; **P < 0.001 when compared to the control mice.

Article Snippet: The Prx1-Cre , Osx-Cre , and floxed Tsc1 ( iDTR , tomato ) mouse lines were originally generated from the Jackson Laboratory.

Techniques: Knock-Out, Injection, Staining, Control

Journal: Scientific Reports

Article Title: Tsc1 ablation in Prx1 and Osterix lineages causes renal cystogenesis in mouse

doi: 10.1038/s41598-018-37139-9

Figure Lengend Snippet: Prx1-Cre; Tsc1 f/f mouse kidney samples showed an increase in proliferating cells and apoptotic cells compared to control mice. ( A ) Immunofluorescent staining for different renal cell markers at the cyst region of Prx1-Cre; Tsc1 f/f mouse revealed that the cysts were mainly positive for THP. ( B ) Representative images of Ki67-stained kidney sections. ( C ) Quantitative analysis of Ki67 positive cells. N = 3. *P < 0.05; **P < 0.001 when compared to the control mice. ( D ) Representative images of TUNEL stained kidney sections. ( E ) Quantitative analysis of TUNEL positive cells. N = 3. *P < 0.05; **P < 0.001 when compared to the control mice.

Article Snippet: The Prx1-Cre , Osx-Cre , and floxed Tsc1 ( iDTR , tomato ) mouse lines were originally generated from the Jackson Laboratory.

Techniques: Control, Staining, TUNEL Assay

Journal: Scientific Reports

Article Title: Tsc1 ablation in Prx1 and Osterix lineages causes renal cystogenesis in mouse

doi: 10.1038/s41598-018-37139-9

Figure Lengend Snippet: Conditional knockout of Tsc1 in the Osx lineage resulted in the development of mTORC1-dependent cysts. ( A ) Osx-Cre; Tsc1 f/ f mouse kidney sections showed increased p-S6 signals. ( B ) Osx-Cre; Tsc1 f/f mice showed enlarged kidneys that were rescued by rapamycin. Left panel: the kidneys of different mouse lines. Right panel: the weights of the kidneys of different mouse lines. ( C ) H-E staining (4x) of the kidney sections of Osx-Cre; Tsc1 f/f and control mice treated with rapamycin or vehicle. ( D ) Images of kidney sections of Osx-Cre; Tsc1 f/f and control mice treated with rapamycin or vehicle. ( E ) Quantitative data of the cystic index of Osx-Cre; Tsc1 f/f and control mice treated with rapamycin or vehicle. The cystic index for control mice is 0. ( F ) Quantitative analysis of glomerulus separation. N = 3. *P < 0.05; **P < 0.001 when compared to the control mice.

Article Snippet: The Prx1-Cre , Osx-Cre , and floxed Tsc1 ( iDTR , tomato ) mouse lines were originally generated from the Jackson Laboratory.

Techniques: Knock-Out, Staining, Control

Journal: Scientific Reports

Article Title: Tsc1 ablation in Prx1 and Osterix lineages causes renal cystogenesis in mouse

doi: 10.1038/s41598-018-37139-9

Figure Lengend Snippet: Osx-Cre; Tsc1 f/f mouse kidney samples showed increased proliferating cells and apoptotic cells compared to control mice. ( A ) Immunofluorescent staining for different renal cell markers at the cyst region of Osx-Cre; Tsc1 f/f mouse showed that the cysts were only positive for Villin. ( B ) Representative images of Ki67-staining kidney sections. ( C ) Quantitative analysis of Ki67 positive cells. ( D ) Representative images of TUNEL staining kidney sections. Arrows indicate apoptotic cells. ( E ) Quantitative analysis of TUNEL positive cells. N = 3. *P < 0.05; **P < 0.001 when compared to the control mice.

Article Snippet: The Prx1-Cre , Osx-Cre , and floxed Tsc1 ( iDTR , tomato ) mouse lines were originally generated from the Jackson Laboratory.

Techniques: Control, Staining, TUNEL Assay

Journal: Scientific Reports

Article Title: Tsc1 ablation in Prx1 and Osterix lineages causes renal cystogenesis in mouse

doi: 10.1038/s41598-018-37139-9

Figure Lengend Snippet: Conditional knockout of Tsc1 in the Dermo1 lineage did not result in cystogenesis. ( A ) Microscopic images of kidney sections of Dermo1-Cre; tdTomato mice showed that Dermo1 could label tubular and glomerulus cells in mouse kidney. ( B ) Immunofluorescent staining on Dermo1-Cre; tdTomato mouse kidney sections revealed that Dermo1 linage renal cells were negative for the tested markers of different renal cell types. ( C ) Dermo1-Cre; Tsc1 f/f mouse kidney sections showed increased p-S6 signals. ( D ) H-E staining (4x) of the kidney sections of 2-month-old Dermo1-Cre; Tsc1 f/f and control mice.

Article Snippet: The Prx1-Cre , Osx-Cre , and floxed Tsc1 ( iDTR , tomato ) mouse lines were originally generated from the Jackson Laboratory.

Techniques: Knock-Out, Staining, Control